FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12509078
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137250.073.15135120
GAGCACA219050.048.89633120
AGAGCAC300850.035.808456120
AAGAGCA466350.023.34656118-119
GAAGAGC526000.021.480007120
GGAAGAG625400.017.789143118-119
TCGGAAG748250.015.136844120
CGGAAGA742650.014.869273118-119
ATCGGAA889400.012.936725120
GATCGGA891550.012.768392118-119
AGATCGG972300.011.520217118-119
TAGATCG238150.011.035358116-117
CTAGATC60350.010.540925120
TTAGATC84550.09.451198114-115
CACACGT34950.09.100784120
GAGATCG397400.09.100012120
GTCTAGA7252.5371264E-88.551592116-117
GCTAGAT23350.08.40811118-119
CGCTAGA7809.811629E-98.392131110-111
CAGATCG391900.08.315496112-113
Produced by FastQC (version 0.11.5)