FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15693245
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188850.077.52541120
GAGCACA298850.046.562504120
AGAGCAC394800.036.35792118-119
AAGAGCA617000.023.840788120
GAAGAGC678150.021.270088116-117
GGAAGAG846450.017.015146118-119
CGGAAGA961700.015.146406114-115
TCGGAAG953650.014.926115116-117
ATCGGAA1124300.012.966102112-113
GATCGGA1137650.012.554818114-115
AGATCGG1234500.011.803424110-111
CTAGATC75650.011.142457120
TAGATCG276500.011.059365112-113
AAGATCG548850.09.335468114-115
GAGATCG420400.08.989897120
CACACGT43900.08.813605120
TTAGATC122700.08.645717108-109
CAGATCG361400.08.125141120
CACGTCT51600.07.7662864118-119
ACACGTC51100.07.571767120
Produced by FastQC (version 0.11.5)