FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12732338
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140150.064.92016120
GAGCACA213800.042.34347120
AGAGCAC283300.034.848183120
AAGAGCA434750.022.319485120
GAAGAGC509000.019.919722120
GGAAGAG608100.016.609303120
CGGAAGA720400.014.196442118-119
TCGGAAG724200.014.027409120
ATCGGAA833200.012.270493116-117
GATCGGA854500.011.945704118-119
AGATCGG959250.010.881735120
TAGATCG228250.010.798995120
CTAGATC61050.010.280171116-117
TTAGATC81450.09.861245114-115
GTCTAGA7103.9746345E-49.160023120
CACACGT42700.08.986262120
GAGATCG409550.07.908194120
ACACGTC55200.07.7760634120
AAGATCG353500.07.616816118-119
CCTAGAT23950.07.6035266118-119
Produced by FastQC (version 0.11.5)