FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11996686
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166300.0102.492645120
GAGCACA282900.061.40623120
AGAGCAC380300.045.414455120
AAGAGCA564800.030.953636118-119
GAAGAGC627050.027.365664116-117
GGAAGAG742600.023.36169116-117
CGGAAGA883750.019.47842114-115
TCGGAAG923350.018.77478114-115
GATCGGA1099500.015.961235112-113
ATCGGAA1090150.015.940382112-113
AGATCGG1209450.014.425362110-111
ACACGTC46900.013.778214120
CACGTCT44250.013.465427120
TAGATCG275350.013.327365110-111
CACACGT39800.013.284112120
ACGTCTG56700.012.432866120
CTAGATC67850.012.3005495110-111
GAGATCG452500.011.78935110-111
TTAGATC98100.011.671185110-111
CAGATCG394850.011.343848110-111
Produced by FastQC (version 0.11.5)