FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505285
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA956300.011.568634120
CGTATCA435700.09.996878120
GCCGTAT561150.09.654374120
TGGTCGC752550.09.598569120
CCGTATC525400.09.3521120
GTATCAT452950.09.112835120
CGCCGTA608450.08.726372120
GGTCGCC721200.08.718081120
GTCGCCG733400.08.712124120
TCATTAA348300.08.578102120
GTGGTCG817600.08.541368120
ATCATTA407900.08.413131120
TCGCCGT703000.08.371996120
GGTGGTC890000.08.29819120
CGGTGGT991750.08.02757120
TCGGTGG1037750.07.9087653120
CTCGGTG1113650.07.789954120
CATTAAA349500.07.6731853120
GATCTCG1334500.07.3954163120
ATCTCGG1246500.07.344759120
Produced by FastQC (version 0.11.5)