FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9660532
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123100.070.01221120
GAGCACA182000.044.891987120
AGAGCAC249050.034.605515120
AAGAGCA390150.023.15056120
GAAGAGC408100.021.431295118-119
GGAAGAG503550.016.711979116-117
CGGAAGA591850.014.99338116-117
TCGGAAG577250.014.435945114-115
CACGTCT34700.013.511429120
ACACGTC34900.012.6437645120
ATCGGAA704250.012.591475114-115
GATCGGA672250.012.514765112-113
CACACGT30800.012.424154118-119
AGATCGG736150.011.976449112-113
CTAGATC35050.011.016223116-117
TAGATCG140300.010.713236120
ACGTCTG46500.010.230997120
AAGATCG375800.09.7339945114-115
TTAGATC67700.09.573286120
GAGATCG217800.09.229418112-113
Produced by FastQC (version 0.11.5)