FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10935832
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC157400.080.93741120
GAGCACA236000.049.00837120
AGAGCAC320550.038.45333120
AAGAGCA476800.025.650719120
GAAGAGC543600.022.513657118-119
GGAAGAG649000.018.931284118-119
CGGAAGA759950.016.099394118-119
TCGGAAG769200.015.935112116-117
CACGTCT46100.015.210237120
CCTAGAT18950.014.021889120
CACACGT44600.013.654006118-119
ATCGGAA913500.013.580132114-115
GATCGGA938350.013.208693114-115
ACACGTC51400.012.205878120
AGATCGG1035600.012.123431120
CTAGATC60150.011.35172116-117
TAGATCG245850.011.244832112-113
ACGTCTG60750.011.056263120
TTAGATC92200.010.047402118-119
CGTCTGA67400.09.417851120
Produced by FastQC (version 0.11.5)