FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644107
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164750.076.07951120
GAGCACA268850.047.97361120
AGAGCAC365950.035.61939120
AAGAGCA540950.024.197798118-119
GAAGAGC601450.021.404428118-119
GGAAGAG729050.017.964006116-117
TCGGAAG878550.015.289764120
CGGAAGA862900.015.058235116-117
ATCGGAA1027600.012.911806118-119
GATCGGA1048550.012.571932112-113
AGATCGG1150950.011.590604116-117
TAGATCG271400.011.198207120
CTAGATC70700.010.188833118-119
CACACGT46700.010.136472120
TTAGATC108100.09.773489120
CACGTCT57800.09.614169120
AAGATCG446850.09.10432120
GAGATCG409500.08.795489116-117
CAGATCG371850.08.163945116-117
ATAGATC125400.07.932739114-115
Produced by FastQC (version 0.11.5)