FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16838831
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC213650.081.74396120
GAGCACA345600.051.368095120
AGAGCAC469800.037.40134118-119
AAGAGCA702000.025.171871118-119
GAAGAGC808750.022.858833120
GGAAGAG984800.018.46582120
CGGAAGA1163550.015.885735118-119
TCGGAAG1193300.015.291289118-119
ATCGGAA1400200.013.171456116-117
GATCGGA1437550.013.127455120
AGATCGG1576200.011.757318118-119
TAGATCG321450.010.588724118-119
CTAGATC88950.010.414984120
AAGATCG689100.09.455997114-115
GAGATCG547700.08.895923118-119
CCTAGAT30500.08.887358116-117
TTAGATC127600.08.79311116-117
TAAGATC273750.08.385559112-113
GCTAGAT34300.08.202959108-109
CACGTCT51400.08.143995120
Produced by FastQC (version 0.11.5)