FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13044310
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG12700.024.241653120
GTCTAGG10300.017.366995114-115
CGTCTAG13600.016.857658120
TCTAGGG12300.014.071619116-117
CTAGGTG7900.012.711884114-115
TCTAGGT10100.012.322543120
GATCTAG13200.010.698567112-113
GTCTAGA14750.010.6381235114-115
ATCTAGG11550.010.324967112-113
CTAGGGA8900.09.657482108-109
CGTAGTG42900.09.314095120
GTGGTCG301400.08.649837120
TGGTCGC277650.08.516824120
TCGGTGG384900.08.4411545120
ATCTCGG464550.08.41802120
CGAGTAG27600.08.300434114-115
CGTATCA181600.08.296217120
TAGATCT524350.08.23251120
CTAGGGT5902.4191067E-68.148819116-117
AGTGTAG679950.08.140439120
Produced by FastQC (version 0.11.5)