FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13044310
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142500.070.589325120
GAGCACA227900.045.781113120
AGAGCAC307350.034.94768120
AAGAGCA464700.022.981636118-119
GAAGAGC534200.020.595299120
GGAAGAG660800.016.485006118-119
TCGGAAG767500.014.509149120
CGGAAGA792650.014.293481120
ATCGGAA893250.012.632529118-119
GATCGGA915550.012.59393120
AGATCGG1016500.011.156693118-119
TAGATCG210000.010.908876118-119
CTAGATC53600.010.653872116-117
CACGTCT44700.09.7255120
TTAGATC81100.08.976598116-117
CACACGT40550.08.906539120
AAGATCG394850.08.652062116-117
TCTAGAT27650.08.224129120
GAGATCG421200.08.18997112-113
ACACGTC49700.07.9396567120
Produced by FastQC (version 0.11.5)