FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18984754
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT288600.08.045045120
GTAGATC375050.07.5823364120
GTGTAGA392950.07.557563120
AGTGTAG380250.07.431293118-119
GAGTGTA405950.07.418994120
AGAGTGT444900.07.376303120
AGATCTC319600.07.264706120
ATCTCGG284850.07.2242327120
AAGAGTG507000.07.20648120
TGTAGGG738300.06.890914120
TGGTCGC159350.06.8898993120
GTAGGGA682350.06.866852120
GTGGTCG188250.06.78826120
TGTAGAT441350.06.783148120
GTGTAGG798950.06.7507796120
TCTCGGT263500.06.705287118-119
GATCTCG338350.06.684809120
CTCGGTG286050.06.6695886120
CGTGTAG824600.06.657201120
GTCGTGT918150.06.651935120
Produced by FastQC (version 0.11.5)