FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465058
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC110000.064.52407120
GAGCACA168650.042.275166120
AGAGCAC225600.032.59802120
AAGAGCA364450.021.374954120
GAAGAGC407400.019.200195120
GGAAGAG507550.015.611303118-119
TCGGAAG589750.014.089769120
CGGAAGA607500.013.519782120
GATCGGA698500.012.116422120
ATCGGAA703900.012.023469120
AGATCGG777300.011.02393118-119
TAGATCG210700.010.402216118-119
CTAGATC48200.09.876392116-117
CACGTCT37700.09.183733120
ACACGTC42450.08.911303120
GCCTAGA8854.8415743E-58.693704120
TTAGATC87900.08.461278120
AAGATCG273400.08.22698118-119
GAGATCG312650.08.185215112-113
CACACGT37650.08.061668118-119
Produced by FastQC (version 0.11.5)