FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24165982
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC403800.0122.405235120
GAGCACA697250.068.22646120
AGAGCAC928900.052.346066118-119
AAGAGCA1355600.034.747837118-119
GAAGAGC1534300.031.602852116-117
GGAAGAG1847100.025.440725116-117
CGGAAGA2132800.022.874346114-115
TCGGAAG2217100.021.114376114-115
ATCGGAA2577900.019.043034112-113
CACGTCT105750.017.819954120
GATCGGA2656750.017.742046112-113
ACACGTC104950.017.687794120
CACACGT98450.017.522377120
AGATCGG2874200.017.063366110-111
TAGATCG733650.015.349632110-111
CTAGATC207650.014.3235235108-109
TTAGATC247200.013.602074108-109
CAGATCG991150.013.23028110-111
GAGATCG1022250.013.125137110-111
AAGATCG761650.012.795954110-111
Produced by FastQC (version 0.11.5)