FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13481464
Sequences flagged as poor quality0
Sequence length54-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2487950.015.625895120
TCATTAA834700.09.736781120
CATTAAA792900.09.734927120
ATCATTA930200.09.705785120
GTATCAT1049250.09.703731120
CGTATCA1102800.09.553903120
GCGTCGG86350.09.460729120
TATCATT1062150.09.156068120
CCGTATC1224550.09.065118120
GCCGTAT1308250.08.769819120
CGCCGTA1402250.08.747386120
TCGCCGT1541700.08.470473120
GTCGCCG1640250.08.401008120
TGGTCGC1825250.08.289987120
TCTCGGG30850.08.177906120
GGTCGCC1714850.08.147635120
ATTAAAA890050.08.145934120
GTGGTCG1957550.08.082598120
TTAAAAA864600.07.579797120
GGTGGTC2085350.07.451874120
Produced by FastQC (version 0.11.5)