FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18030594
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166750.060.935734120
GAGCACA250600.041.31621120
AGAGCAC335200.032.767555120
AAGAGCA571950.020.159065120
GAAGAGC612400.018.491695120
GGAAGAG816550.014.69493120
TCGGAAG883000.013.729575118-119
CGGAAGA965250.012.877235120
ATCGGAA1088250.011.900148120
AGATCGG1155050.011.690452120
GATCGGA1083750.011.505968118-119
TAGATCG261350.010.700892118-119
ACACGTC62700.09.942834120
CTAGATC70800.09.440742120
AAGATCG514000.08.690159120
GAGATCG407550.08.484132120
TTAGATC117150.07.9119143118-119
CACACGT58050.07.9005947118-119
CACGTCT60500.07.754852120
CAGATCG397550.07.5510974118-119
Produced by FastQC (version 0.11.5)