FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13941083
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163250.070.1622120
GAGCACA248800.047.983654120
AGAGCAC337600.035.84733120
AAGAGCA525700.023.20252120
GAAGAGC568100.021.843063120
GGAAGAG737700.017.191162120
TCGGAAG861950.014.607004118-119
CGGAAGA887700.014.40185118-119
GATCGGA1020750.012.370904116-117
ATCGGAA1041750.012.337382120
AGATCGG1099050.011.557692114-115
TAGATCG277550.010.840715114-115
CTAGATC61650.010.324176116-117
AAGATCG405750.09.176402110-111
GAGATCG393550.09.031158120
TTAGATC99400.08.697495116-117
ACACGTC41050.08.475449120
CAGATCG355000.07.917253120
CCTAGAT22400.07.9013667114-115
ATAGATC158150.07.8506904120
Produced by FastQC (version 0.11.5)