FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125110
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173150.063.877388120
GAGCACA260650.042.257095120
AGAGCAC343250.033.58346120
AAGAGCA524300.022.26259120
GAAGAGC587650.019.875965118-119
GGAAGAG735800.016.462505120
TCGGAAG836800.014.29573118-119
CGGAAGA854750.013.925198120
ATCGGAA1012350.012.790759120
GATCGGA1036550.012.327099120
AGATCGG1152050.011.251148120
CACGTCT52750.010.976455120
TAGATCG263850.010.347337114-115
CACACGT53000.09.559095120
ACACGTC59900.08.897338120
CTAGATC73150.08.623776116-117
AAGATCG513000.08.388075120
GAGATCG413000.07.9656615120
TTAGATC117850.07.9279456120
CAGATCG409300.07.192396118-119
Produced by FastQC (version 0.11.5)