FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13651165
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146750.069.321014120
GAGCACA229650.044.41274120
AGAGCAC304200.033.52883118-119
AAGAGCA477950.021.825485120
GAAGAGC527100.019.344439116-117
GGAAGAG636000.016.69367120
TCGGAAG749400.014.061378120
CGGAAGA753400.013.969116120
GATCGGA879250.012.090803116-117
ATCGGAA886800.012.0884695118-119
AGATCGG968700.011.066758116-117
CTAGATC62050.010.42046110-111
TAGATCG259050.010.34252114-115
TTAGATC109150.09.417283120
AAGATCG366250.08.609967114-115
GAGATCG332100.08.317258116-117
CACGTCT49300.07.5328403120
CACACGT43800.07.4946675118-119
CAGATCG330500.07.4442983118-119
ACACGTC50850.07.303226120
Produced by FastQC (version 0.11.5)