FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11726043
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC108750.065.03143120
GAGCACA177400.042.496937120
AGAGCAC237750.034.23776120
AAGAGCA384400.021.608433120
GAAGAGC424000.019.906986120
GGAAGAG515800.016.475595120
TCGGAAG601000.014.608092120
CGGAAGA604400.014.240265120
GATCGGA722450.013.099399120
ATCGGAA707850.012.890809120
AGATCGG809800.011.749588120
TAGATCG199950.011.081059118-119
CTAGATC53400.010.177873116-117
TTAGATC85850.09.310375120
AAGATCG317800.08.39038118-119
GAGATCG321400.08.176989120
CCTAGAT24200.07.926569114-115
CAGATCG284150.07.898723120
GTAGATC74050.07.512624120
ATAGATC94900.07.007524120
Produced by FastQC (version 0.11.5)