FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17584056
Sequences flagged as poor quality0
Sequence length53-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG31050.019.126741120
AAAAAAA15581450.015.743376120
GGTCCCC4801.6887903E-512.5055521
GACCCCC1750.003251360311.99894616-17
CGGTGGG25900.011.580775120
GGTCCCG11101.8189894E-1211.3563931
GGTCCCA12652.5465852E-119.9648981
TCTAGGT14300.09.019263104-105
GGTCCGC12853.597961E-98.8755361
TTAAAAA3846650.08.783091120
CGGGGGT10952.2883614E-78.76543120
CTAGGTG10350.08.693986108-109
ATTAAAA4026100.08.677691120
TAAAAAA3768000.08.635275120
GGTCCAC12552.2622771E-88.60941
CATTAAA4039500.08.605839120
GGGCTCG9251.9080115E-58.4361781
TCATTAA4295850.08.231937120
GGTCGAC18000.08.16509118-119
AAAAAAC804050.07.968115118-119
Produced by FastQC (version 0.11.5)