FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003474
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC162050.077.66625120
GAGCACA264850.048.244617120
AGAGCAC357250.036.283424120
AAGAGCA532800.024.555176120
GAAGAGC620100.021.728176120
GGAAGAG736350.018.172483118-119
CGGAAGA871700.015.375261120
TCGGAAG870350.015.346961116-117
ATCGGAA1019200.013.1509116-117
GATCGGA1059250.012.780331120
AGATCGG1159950.011.695311120
CACGTCT54700.011.296648120
TAGATCG276800.010.751754110-111
CTAGATC56350.010.147046112-113
CACACGT47950.010.072516118-119
GAGATCG525650.09.256176112-113
ACACGTC59500.09.251292118-119
TTAGATC96450.09.020934118-119
CCTAGAT19700.09.013414108-109
ACGTCTG70200.08.498843120
Produced by FastQC (version 0.11.5)