FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15233210
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC276850.0158.69128120
GAGCACA456400.093.65228120
AGAGCAC612850.070.605225118-119
AAGAGCA896150.046.902824118-119
GAAGAGC995350.043.295677116-117
GGAAGAG1202900.034.702263116-117
CGGAAGA1423250.030.52572114-115
CACGTCT81250.028.694536120
TCGGAAG1461750.028.553566114-115
ACACGTC84300.027.210287120
ATCGGAA1704450.025.764925112-113
CACACGT82900.024.521063118-119
GATCGGA1749300.024.181835112-113
AGATCGG1855000.023.505177110-111
TAGATCG465500.020.423786110-111
CTAGATC96400.019.91928110-111
ACGTCTG106700.019.73579120
GAGATCG620950.019.450823110-111
GCACACG99150.019.303637118-119
CGTCTGA122050.019.204962120
Produced by FastQC (version 0.11.5)