FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15142050
Sequences flagged as poor quality0
Sequence length51-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG92350.011.762088120
CGTATCA323700.09.90014120
CGCCGTA444200.09.281559120
TGGTCGC552250.09.193426120
GGTGGTC656700.09.129379120
GCCGTAT417050.09.008012120
GTGGTCG599400.08.990883120
GGTCGCC531500.08.953909120
TCGGTGG778350.08.766542120
CGGTGGT732650.08.756367120
GTCGCCG541900.08.615951120
CCGTATC385400.08.486479120
GTATCAT351300.08.456094120
CTCGGTG835200.08.4285120
TCTCGGT886300.08.287879120
ATCTCGG961950.08.060329120
AGATCTC1080300.07.9883533120
TAGATCT1137250.07.8416166120
TCGCCGT510850.07.8239055120
AAAAAAA1117700.07.817698120
Produced by FastQC (version 0.11.5)