FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15128524
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC223000.0103.05225120
GAGCACA351200.064.40766120
AGAGCAC479550.047.615498118-119
CGATCTA46550.041.8710025
CCGATCT160750.040.6890454
CTTCCGA184700.036.1086921
TCCGATC190850.034.602083
AAGAGCA730750.031.194523118-119
GAAGAGC820300.028.035458116-117
TTCCGAT262100.025.7169112
GGAAGAG1060700.021.871538116-117
GATCTAG15350.020.5353456
CGGAAGA1234200.019.054932114-115
TCGGAAG1252300.018.46773114-115
ATCGGAA1475000.016.031965112-113
GATCGGA1521550.015.3187112-113
CACACGT59850.014.922631120
AGATCGG1605650.014.534497110-111
CACGTCT86200.013.8868685118-119
TAGATCG490000.013.759156110-111
Produced by FastQC (version 0.11.5)