FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11337576
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130200.065.28308120
GAGCACA201000.042.552143120
AGAGCAC256600.033.37969118-119
AAGAGCA402750.023.066612120
GAAGAGC445450.019.927198118-119
GGAAGAG550250.016.649553118-119
CGGAAGA657300.014.659037120
TCGGAAG648950.014.270407118-119
ATCGGAA780050.012.318213120
GATCGGA791050.011.945609118-119
AGATCGG857400.011.137311116-117
CTAGATC56550.010.80331120
TAGATCG218950.010.342155120
ACACGTC47950.010.109645120
TTAGATC82050.08.637882110-111
AAGATCG340750.08.457764120
GAGATCG314750.08.312509120
CCTAGAT19500.07.491861120
CACACGT40650.07.333181118-119
CAGATCG311600.07.288368120
Produced by FastQC (version 0.11.5)