FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17358203
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178850.071.00833120
GAGCACA275650.044.52309120
AGAGCAC368850.034.039043120
AAGAGCA580850.021.547806118-119
GAAGAGC642300.020.856705120
GGAAGAG789150.016.525986120
CGGAAGA963300.014.145347120
TCGGAAG945800.014.042567118-119
GATCGGA1108050.012.463492120
ATCGGAA1104600.012.407253120
TAGATCG299350.011.368858120
AGATCGG1221100.011.2721118-119
CTAGATC72700.09.037278118-119
GAGATCG502200.08.915673112-113
CAGATCG436350.08.303909118-119
AAGATCG385850.08.258281120
TTAGATC104350.08.122026116-117
GTAGATC95500.07.5332575118-119
TAAGATC161350.07.451583120
AGAGATC190600.07.2043023114-115
Produced by FastQC (version 0.11.5)