FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18241852
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC188250.064.10768120
GAGCACA286650.041.875084120
AGAGCAC373750.032.86165120
AAGAGCA582100.021.094486118-119
GAAGAGC657200.019.40798120
GGAAGAG804200.015.8362255120
CGGAAGA960050.014.297804120
TCGGAAG945600.014.194318120
GATCGGA1091950.012.366361118-119
ATCGGAA1117350.012.259174118-119
AGATCGG1212850.011.256633116-117
TAGATCG299900.010.379226116-117
CTAGATC73350.09.449165108-109
GAGATCG464400.08.8994120
TTAGATC117400.08.8839445116-117
AAGATCG443950.08.718703116-117
CAGATCG380750.07.99632120
ACACGTC71800.07.668788120
TAAGATC157050.07.5069876120
CACGTCT66000.07.459366120
Produced by FastQC (version 0.11.5)