FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11308410
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC145650.085.53729120
GAGCACA239950.053.41004120
AGAGCAC324700.039.011066120
AAGAGCA492000.026.335659120
GAAGAGC545750.023.454203118-119
GGAAGAG671750.019.154099116-117
CGGAAGA779600.016.480452116-117
TCGGAAG788050.016.18589114-115
ATCGGAA921100.013.993316114-115
GATCGGA949350.013.491449112-113
AGATCGG1026900.012.516077112-113
CACGTCT46850.012.390691120
TAGATCG255600.011.326592120
CTAGATC51450.010.486538114-115
CACACGT41950.010.289209118-119
GAGATCG415800.09.8711195110-111
ACACGTC51700.09.572318118-119
AAGATCG306050.09.34995110-111
CAGATCG359050.09.327422112-113
TTAGATC86350.09.049662108-109
Produced by FastQC (version 0.11.5)