FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16356089
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC182550.078.62275120
GAGCACA321550.043.779644120
AGAGCAC428050.035.85123120
AAGAGCA668750.023.128157120
GAAGAGC758150.020.44518118-119
GGAAGAG913550.017.378855120
CGGAAGA1025700.015.334663120
TCGGAAG1048500.015.061422118-119
ATCGGAA1218700.012.84531118-119
GATCGGA1234200.012.79849120
AGATCGG1361350.011.484728116-117
TAGATCG324100.010.832907120
CTAGATC90800.010.054845120
AAGATCG554700.09.624604118-119
GAGATCG454600.09.423867112-113
TTAGATC127600.08.977446114-115
CAGATCG375800.08.672282114-115
TAAGATC216550.08.494047120
ACTAGAT34800.07.5232887120
TGAGATC194200.07.4677787118-119
Produced by FastQC (version 0.11.5)