FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15903389
Sequences flagged as poor quality0
Sequence length65-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC9000.025.331253120
AGCCCTA26850.014.7477161
GCGTCGG111900.014.476022120
GCCCTAA29050.013.2182082
CCTTCAT108100.012.6541881
TCTCGGG39950.010.662454120
CCTTCAC55850.010.5275671
GTTCCCT64350.010.4422631
GGCCTAA15000.010.19925876-77
AAAAAAA1243100.010.086855120
TGGTCGC778850.09.690415120
CCGTATC555300.09.561612120
CGTATCA480450.09.527784120
CCTTTAT187500.09.1834471
GGTCGCC733000.09.077017120
GTGGTCG840350.09.016931120
GGTGGTC914250.08.944302120
CGCCGTA657650.08.921947120
CATTCAT147600.08.8206071
GTCGCCG754100.08.775304120
Produced by FastQC (version 0.11.5)