FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15903389
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC269600.0106.48205120
GAGCACA460500.062.44167120
AGAGCAC639300.045.197697118-119
CCTAGAC8850.040.56945120
AAGAGCA947250.029.97662120
GAAGAGC1062200.027.408617120
GGAAGAG1271500.022.7987118-119
CACACGT50900.019.474686120
CGGAAGA1507300.019.304598114-115
TCGGAAG1625750.017.842878116-117
CACGTCT71400.015.632282120
ATCGGAA1900750.015.394833112-113
GATCGGA1945650.014.991426114-115
ACACGTC70400.014.413029120
AGATCGG2140800.013.648543110-111
TAGATCG430900.012.869811112-113
ACGTCTG82100.012.739318120
GCACACG73750.012.594169120
GGCCCCT15950.012.478547110-111
GAGATCG917000.012.231282112-113
Produced by FastQC (version 0.11.5)