FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119991
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166000.069.65664120
GAGCACA250650.044.993004120
AGAGCAC332450.034.434578118-119
AAGAGCA501650.024.432123120
GAAGAGC552450.021.318628118-119
GGAAGAG675400.017.60334120
CGGAAGA793000.015.31855120
TCGGAAG804300.014.917393120
ATCGGAA956500.012.849685118-119
CACACGT45700.012.792297120
GATCGGA976850.012.449774118-119
AGATCGG1078950.011.381591116-117
TAGATCG234200.010.971631120
CACGTCT48650.010.898784120
ACACGTC49750.010.657806120
TTAGATC93050.010.300755120
CTAGATC63750.010.129265110-111
GCTAGAT24400.09.472299120
ACGTCTG62350.09.158179120
AAGATCG421150.09.135786120
Produced by FastQC (version 0.11.5)