FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15749039
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC170050.069.3918120
GAGCACA262500.044.393616120
AGAGCAC347200.033.727093118-119
AAGAGCA543500.021.834003120
GAAGAGC605850.019.752148120
GGAAGAG743650.016.118969118-119
TCGGAAG857900.014.030656118-119
CGGAAGA882650.013.988619120
GATCGGA1001650.012.049706116-117
ATCGGAA1007400.011.938527120
AGATCGG1104150.011.182407120
TAGATCG256900.010.6842112-113
CTAGATC66650.010.10782112-113
AAGATCG447150.08.927807114-115
TTAGATC102600.08.646916120
GAGATCG380850.08.319488120
CACGTCT55750.08.255838120
CAGATCG410850.08.012382118-119
CCTAGAT24900.07.902703108-109
CACACGT52450.07.757863118-119
Produced by FastQC (version 0.11.5)