FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16372786
Sequences flagged as poor quality0
Sequence length54-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC145050.09.805499120
GTGGTCG157600.09.481613120
TAGATCT308750.09.271511120
GCGTCGG63800.09.03004120
ATCTCGG265850.08.826311120
AGTGTAG411700.08.818883120
GAGTGTA444350.08.684102120
GATCTCG299600.08.452978120
GGTCGCC146700.08.386154120
AGAGTGT496050.08.323425120
TCTCGGT248700.08.156054120
CTCGGTG236900.08.106325120
AAGAGTG558400.08.028128120
AGATCTC317500.07.9386134120
GTAGATC408100.07.882016120
TAGGGAA766100.07.8726277120
GGTGGTC190600.07.6963296118-119
GTAGGGA795950.07.682941120
GTGTAGA411550.07.524301120
GTGTAGG922550.07.350682120
Produced by FastQC (version 0.11.5)