FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16372786
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151650.059.648003120
GAGCACA219600.039.54022120
AGAGCAC292950.031.767977120
GAAGAGC533700.019.547235120
AAGAGCA504000.019.361256120
GGAAGAG692350.014.640374118-119
TCGGAAG758950.013.720635120
CGGAAGA796500.012.953184118-119
GATCGGA927800.012.519445120
ATCGGAA934050.011.638865120
AGATCGG999400.011.488851120
TAGATCG219400.010.779499118-119
CTAGATC57400.09.974120
AAGATCG504750.08.792448118-119
CACACGT43850.08.413896120
GAGATCG349800.08.037873120
TTAGATC104100.07.806764116-117
CAGATCG343250.07.171981120
ACACGTC46400.06.7176414120
GCTAGAT29650.06.638133118-119
Produced by FastQC (version 0.11.5)