FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12057429
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT265800.010.272138120
AGTGTAG366600.09.44469120
GAGTGTA395650.08.766396120
AGATCTC265400.08.682299120
AGAGTGT437550.08.310924120
GATCTCG263300.08.295735120
CTCGGTG225300.08.096863120
GCGTCGG60400.08.047342120
GTAGATC363400.07.909608120
AAGAGTG502200.07.76678120
GTGTAGA366600.07.578668120
TGTAGGG748700.07.5660386120
TCTCGGT233700.07.549062120
TAGGGAA681250.07.5488133120
GGTCGCC147900.07.4654164120
CGTGTAG860350.07.351396120
ATCTCGG242800.07.3145256118-119
GTAGGGA704750.07.1939063118-119
TGGTCGC146000.07.192661120
GTGGTCG156500.07.170206120
Produced by FastQC (version 0.11.5)