FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15421435
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204500.069.92148120
GAGCACA309100.045.354164120
AGAGCAC408400.035.31307120
AAGAGCA614350.023.586134120
GAAGAGC690850.021.1424120
GGAAGAG819300.018.269423120
CGGAAGA981900.015.348372120
TCGGAAG999700.014.992827118-119
ATCGGAA1183300.013.3535185120
GATCGGA1188750.012.898099118-119
AGATCGG1326550.011.560834118-119
TAGATCG315200.011.030873114-115
CACACGT61550.010.983338120
CTAGATC82550.010.570375108-109
CACGTCT65500.010.008228120
ACACGTC74500.09.532445120
AAGATCG476150.09.393442120
GAGATCG507000.09.360609120
TTAGATC130450.09.160543120
CCTAGAT28550.08.835305114-115
Produced by FastQC (version 0.11.5)