FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12143492
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133050.069.405975120
GAGCACA216950.042.137157120
AGAGCAC276800.033.577053120
AAGAGCA418100.023.418602120
GAAGAGC489800.020.978458120
GGAAGAG584250.017.575727120
CGGAAGA699050.014.795273118-119
TCGGAAG713200.014.360772120
GATCGGA849600.013.01494120
ATCGGAA824600.012.806577120
AGATCGG948400.011.459862118-119
CTAGATC56400.010.696037120
TAGATCG208350.010.636154118-119
TTAGATC77800.010.395391120
AAGATCG394600.08.651886120
GCTAGAT21750.08.534133120
CAGATCG336950.08.333848118-119
GAGATCG377050.08.123037116-117
CCTAGAT18350.07.5865417120
ACACGTC47450.07.1251593120
Produced by FastQC (version 0.11.5)