FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18167499
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC175450.063.0476120
GAGCACA282600.040.763237120
AGAGCAC361750.031.193989118-119
AAGAGCA571550.021.101166120
GAAGAGC664100.018.304152120
GGAAGAG793650.015.837269120
TCGGAAG920800.013.899046120
CGGAAGA937050.013.773416120
ATCGGAA1064450.012.985444120
GATCGGA1049000.012.855314120
AGATCGG1173100.011.820717120
TAGATCG302850.011.173943120
CTAGATC81450.010.297204118-119
TTAGATC118300.09.372321118-119
GAGATCG423700.09.08279120
AAGATCG452100.08.892113120
CAGATCG382450.08.381524118-119
CCTAGAT30200.07.7932234120
TCTAGAT41050.07.282945120
TGAGATC184600.06.9011135118-119
Produced by FastQC (version 0.11.5)