FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13019535
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC205900.0105.12353120
GAGCACA345050.063.155613120
AGAGCAC470350.046.211857118-119
AAGAGCA704600.030.513166118-119
GAAGAGC782150.027.840595120
GGAAGAG965700.022.717983120
CGGAAGA1113500.019.74327118-119
CACGTCT42750.018.709953120
TCGGAAG1156600.018.468956114-115
ACACGTC45200.017.154099120
CACACGT43800.017.14338120
ATCGGAA1361600.016.241505116-117
ACGTCTG53450.015.880654120
GATCGGA1420350.015.176688112-113
AGATCGG1554950.014.203668114-115
TAGATCG372950.013.07987114-115
GCACACG62500.012.275257120
GAGATCG629750.011.715976112-113
CTAGATC60250.011.219595108-109
CAGATCG471450.011.132898110-111
Produced by FastQC (version 0.11.5)