FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318143
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC95050.055.267143120
GAGCACA150500.038.420475120
AGAGCAC197750.030.433186120
AAGAGCA302350.019.777903118-119
GAAGAGC353550.018.140074120
GGAAGAG415850.015.560419120
TCGGAAG466950.013.912197120
CGGAAGA491000.013.165695118-119
ATCGGAA547850.012.428008120
GATCGGA554850.012.075887120
AGATCGG628250.011.192702120
TAGATCG144500.010.323809120
CTAGATC40850.09.441536116-117
AAGATCG255800.09.22132120
CACACGT33850.08.75753118-119
TTAGATC62900.08.260055112-113
GAGATCG238400.07.862120
ACACGTC49250.07.378372120
CCCTAGA7903.9746192E-77.2625866114-115
ACGTCTG47750.07.209619120
Produced by FastQC (version 0.11.5)