FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14426397
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127650.065.62672120
GAGCACA196950.040.973995120
AGAGCAC256950.032.027798118-119
AAGAGCA425300.020.103868120
GAAGAGC445700.018.996891120
GGAAGAG550400.015.848656120
TCGGAAG638100.013.851094120
CGGAAGA651550.013.339079120
GATCGGA756150.011.968175120
ATCGGAA766500.011.681234120
AGATCGG823200.011.0011425120
TAGATCG215100.010.421288120
CTAGATC53350.09.123739120
AAGATCG377300.08.5892935120
TTAGATC107950.08.115122116-117
GAGATCG292450.07.83853118-119
CAGATCG281250.07.1048646120
TAAGATC172800.06.1797347118-119
ATAGATC120350.05.737723112-113
TGAGATC143350.05.7188144120
Produced by FastQC (version 0.11.5)