FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12743571
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC104050.038.35893120
GAGCACA147700.028.108576120
AGAGCAC179300.024.97817120
AAGAGCA282100.015.897748120
GAAGAGC296350.015.258202118-119
GGAAGAG380900.013.490819120
TCGGAAG389400.013.196335118-119
CGGAAGA437250.011.9074120
GATCGGA466000.011.10658116-117
ATCGGAA486950.010.882109120
AGATCGG520500.010.8443775120
TAGATCG105950.09.898109114-115
CTAGATC32550.08.907404110-111
CCTAGAT14900.08.694354112-113
TTAGATC50600.07.9244137120
AAGATCG231900.07.12916120
GAGATCG267700.06.7518616120
GCTAGAT17003.6379788E-126.5317245120
CAGATCG252200.05.5279956120
GCCTAGA9651.1038913E-45.4337034110-111
Produced by FastQC (version 0.11.5)