FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13300590
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152750.075.32655120
GAGCACA241650.047.14751120
AGAGCAC325150.035.254646118-119
AAGAGCA494700.023.567669120
GAAGAGC566050.020.620462120
GGAAGAG685550.017.752836120
CGGAAGA823800.015.160637120
TCGGAAG841900.015.102987120
ATCGGAA989250.012.846774118-119
GATCGGA1005950.012.824906120
AGATCGG1116350.011.354374116-117
TAGATCG269400.011.170742120
CTAGATC65500.010.953776120
CACACGT39800.010.181885120
TTAGATC97900.08.991137112-113
GAGATCG458300.08.987176120
GCTAGAT24350.08.457615116-117
AAGATCG399050.08.407159118-119
CCTAGAT21050.08.205526118-119
TGAGATC160650.08.105069120
Produced by FastQC (version 0.11.5)