FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12065696
Sequences flagged as poor quality0
Sequence length54-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC374250.08.496778120
TAGATCT274650.08.466209118-119
GATCTCG304550.08.353096120
AGTGTAG373850.08.313283120
GAGTGTA405100.08.13112120
AGAGTGT444350.08.088014120
AGATCTC285950.07.9942017120
GTGTAGA372000.07.806253120
GGTGGTC191750.07.728617120
AAGAGTG501700.07.4743967120
ATCTCGG276200.07.450933120
GTAGGGA712600.07.4177184120
TGGTCGC157350.07.282942120
GGGAAAG664600.07.1951227120
TAGGGAA682000.07.1787033120
GTGGTCG170000.07.164524120
TGTAGGG751350.07.0670996120
CTCGGTG251850.07.0278134120
TGTAGAT404100.07.0079885120
TCGGTGG223050.06.9937696120
Produced by FastQC (version 0.11.5)