FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11409215
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC104050.054.098137120
GAGCACA149400.039.360657120
AGAGCAC203850.031.531277120
AAGAGCA323000.019.919317120
GAAGAGC352800.018.183308120
GGAAGAG445100.015.755008120
TCGGAAG501750.014.414906120
CGGAAGA535250.013.794716120
GATCGGA620650.012.616065120
ATCGGAA612150.012.205622120
AGATCGG700900.011.889434120
TAGATCG179600.010.540507120
CTAGATC45850.010.287818120
TTAGATC72400.010.076756120
CCTAGAT20300.08.9846945120
GAGATCG337500.08.422965118-119
GCTAGAT18250.07.581605120
AAGATCG258950.07.3834443120
CACACGT27300.07.1416636118-119
CAGATCG285850.06.798633120
Produced by FastQC (version 0.11.5)