FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17471467
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC233400.081.552765120
GAGCACA364300.051.235527120
AGAGCAC491400.038.5547120
AAGAGCA737500.026.05976120
GAAGAGC829550.022.82972118-119
GGAAGAG988050.019.074116-117
CGGAAGA1163650.016.36703116-117
TCGGAAG1188800.015.787657114-115
CACACGT67800.014.816115120
CACGTCT69100.014.644267120
ATCGGAA1396450.013.8446865114-115
GATCGGA1428000.013.293115114-115
ACACGTC79550.012.72054120
AGATCGG1577700.012.274845112-113
TAGATCG379250.011.052181112-113
ACGTCTG86100.010.637601120
CTAGATC102850.010.448781110-111
CCCCCCG10856.548362E-1110.2311331
GAGATCG615000.09.590066114-115
CAGATCG586950.09.532097110-111
Produced by FastQC (version 0.11.5)