FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11680525
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC115200.063.488388120
GAGCACA175600.042.826023120
AGAGCAC239700.032.58441120
AAGAGCA376550.020.82786120
GAAGAGC405500.020.374737120
GGAAGAG501800.016.348963120
TCGGAAG597050.014.875120
CGGAAGA600250.013.823287118-119
GATCGGA695850.012.521993118-119
ATCGGAA705500.012.305005118-119
AGATCGG784050.011.203845120
TAGATCG211700.011.165724118-119
CTAGATC50050.010.309074120
CACACGT33600.09.213735120
CACGTCT39900.09.05209120
TTAGATC90300.08.928415116-117
AAGATCG282100.08.710752120
GAGATCG281350.08.585331116-117
CAGATCG256250.08.2555065120
ATAGATC108100.07.3087673118-119
Produced by FastQC (version 0.11.5)