FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13975975
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC179100.089.9134120
GAGCACA302850.051.993164120
AGAGCAC404200.040.798187120
AAGAGCA614450.026.438759118-119
GAAGAGC710150.023.378584120
GGAAGAG845100.019.892038120
CGGAAGA979100.017.150969118-119
TCGGAAG1003650.016.353123118-119
CCCCCCC2504.649238E-415.5268631
ATCGGAA1170050.014.448116-117
GATCGGA1202500.013.711383116-117
AGATCGG1320800.012.74306114-115
CTAGATC87700.011.801017112-113
TAGATCG302000.011.77261114-115
CACGTCT51700.010.9442625120
TTAGATC103250.010.599631120
GAGATCG561150.010.276534112-113
CCCCCCG6659.194318E-610.0065711
CCTAGAT31650.09.880519112-113
CAGATCG497650.09.575472112-113
Produced by FastQC (version 0.11.5)